Protocols
System Suitability (CompRef) Study

Dataset: CompRef_Proteome_VU_B3_P5

Analytical Sample Protocol

  1. Starting Amount 200 µg
  2. Proteolysis Trypsin
  3. Alkylation Iodoacetamide
  4. Enrichment N/A
  5. Labelling Label-Free
  6. Fractionation Peptide Basic pH Reversed Phase
  7. Fractions 15


Chromatography Protocol

  1. Column Type C18
  2. Column Length 11 cm
  3. Inside Diameter 100 µm
  4. Particle Type 300 Å, Phenomenex
  5. Particle Size 5 µm
  6. Injected 2 µl (~2-4µg/µl)
  7. Gradient Length 95 min


Mass Spectrometry Protocol

  1. Type Orbitrap Mass Spectrometry Protocol
  2. Instrument LTQ Orbitrap Velos
  3. Dissociation Collision-induced dissociation
  4. MS 1 Resolution 60,000
  5. Collision Energy 35%
  6. Precursors Top 8