Protocols
System Suitability (CompRef) Study
Dataset: CompRef_Proteome_WU_Ellis-041
Analytical Sample Protocol
-
Starting Amount
450 µg/gel lane; 140 µg digested/MW fraction
-
Proteolysis
Lys-C ; Trypsin
-
Alkylation
Iodoacetamide
-
Enrichment
N/A
-
Labelling
Label-Free
-
Fractionation
Gel-eluted liquid fraction entrapment electrophoresis (GELFrEE)
-
Fractions
7
-
Column Type
C18
-
Column Length
15 cm
-
Inside Diameter
200 µm
-
Particle Type
ChromXP C-18-CL
-
Particle Size
3 µm
-
Injected
15 µg
Mass Spectrometry Protocol
-
Type
High-resolution quadrupole-TOF Mass Spectrometer (Qq-TOF)
-
Instrument
TripleTOF 5600
-
Dissociation
Collision-induced dissociation
-
MS 1 Resolution
30,000
-
MS 2 Resolution
15,000
-
Collision Energy
rolling collision energy (15-45 eV)
-
Precursors
Top 50