Protocols
System Suitability (CompRef) Study

Dataset: CompRef_Proteome_WU_Ellis-041

Analytical Sample Protocol

  1. Starting Amount 450 µg/gel lane; 140 µg digested/MW fraction
  2. Proteolysis Lys-C ; Trypsin
  3. Alkylation Iodoacetamide
  4. Enrichment N/A
  5. Labelling Label-Free
  6. Fractionation Gel-eluted liquid fraction entrapment electrophoresis (GELFrEE)
  7. Fractions 7


Chromatography Protocol

  1. Column Type C18
  2. Column Length 15 cm
  3. Inside Diameter 200 µm
  4. Particle Type ChromXP C-18-CL
  5. Particle Size 3 µm
  6. Injected 15 µg


Mass Spectrometry Protocol

  1. Type High-resolution quadrupole-TOF Mass Spectrometer (Qq-TOF)
  2. Instrument TripleTOF 5600
  3. Dissociation Collision-induced dissociation
  4. MS 1 Resolution 30,000
  5. MS 2 Resolution 15,000
  6. Collision Energy rolling collision energy (15-45 eV)
  7. Precursors Top 50